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| Registros recuperados: 14 | |
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| Zhang,Zuofa; Lv,Guoying; Pan,Huijuan; Santos,Leandro Freire dos; Fan,Menghai; Soccol,Carlos Ricardo; Thomaz-Soccol,Vanete; Lacerda,Luiz Gustavo; Carvalho Filho,Marco Aurélio da Silva; Fan,Leifa. |
| The study aimed to investigate the antihyperglycemic and antilipidperoxidative effects of ethanol extract of the dry matter from culture broth of Coriolus versicolor (ECBC) in streptozotocin-induced diabetes mice. Blood glucose level, insulin level, total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol(LDL-C), high density lipoprotein -cholesterol (HDL-C) in serum and reduced glutathione level (GSH), lipid peroxidation, glycogen, antioxidant enzymes in liver were evaluated. Moreover, histopathological observation was conducted. Streptozotocin treatment (150 mg/kg body weight) induced the decrease of GSH level, antioxidant enzymes activities, glycogen content in liver, HDL-C content and insulin level in serum, accompanied by the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coriolus versiolor; Antihyperglycemic; Antilipidperoxidative; Diabetes. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400008 |
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| Thomaz-Soccol,Andréa; Mocellin,Marcos; Mulinari,Fabiane; Castro,Edilene Alcântara de; Queiroz-Telles,Flávio de; Alcântara,Fernanda de Souza; Bavaresco,Marcello Tortelli; Hennig,Luciane; Andraus,Anielo; Luz,Ennio; Thomaz-Soccol,Vanete. |
| The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mucocutaneous leishmaniasis; Molecular diagnosis; Leishmania (Viannia) braziliensis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008 |
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| Szargiki,Regiane; Castro,Edilene Alcântara de; Luz,Ennio; Kowalthuk,Wolodymir; Machado,Ângela Maria; Thomaz-Soccol,Vanete. |
| We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared: clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-endemic area and carriers of other etiologies. Two antigens were prepared from promastigotes of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis for indirect immunofluorescence assay, ELISA and immunoblotting. The parasitological approaches detected 79.3% of the patients with a clinical diagnosis; the parasites were identified by PCR as L. (V.) braziliensis. Serological methods showed 95% sensitivity for homologous antigens. Immunoblotting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cutaneous leishmaniasis; Immunodiagnosis; Antigen; Western blotting. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100011 |
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| Teodoro,Ueslei; Balduíno,João; Thomaz-Soccol,Vanete; Barbosa,Orlando C; Ferreira,Maria E. M. C; Lozovei,Ana Leuch; Verzignassi,Thaís G; Roberto,Andrea C. B. Silva. |
| Studies were conducted to compare the results of the collections of phlebotomines, in two distinct periods, i.e. before and after changed environmental conditions in Jussara county, Paraná State, Brazil. Collections were made near to a forest in Jussara farm, between February and April 1992. Six Falcão light traps were installed in houses and animals' shelters. Catches were made once a month and 35,783 phlebotomines were captured. Based on these data, sanitation was done in peridomiciliary area and domestic animals shelters were separated from domiciles in January 1994. Then during February to April 1994 using the same methodology new phlebotomines catches were made. The results showed that an average of just 150 (6,311/42) phlebotomines per hour were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Psychodidae; Phlebotomine; Sandflies; Ecology; Lutzomyia. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300007 |
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| Kopp,Rogério Luiz; Thomaz-Soccol,Vanete; Klisiowicz,Débora do Rocio; Membrive,Norberto; Barata,José Maria Soares; Jurberg,José; Steindel,Mário; Kopp,Denize Cristina Trevisan; Castro,Edilene Alcântara de; Luz,Ennio. |
| Panstrongylus megistus is an important Chagas Disease vector and is said to be one of the species that might replace Triatoma infestans as the main vector of that disease in Brazil. The different degrees of P. megistus domiciliation in Brazil and its epidemiological relevance draw forth the need for the development of genetic studies that make it possible to analyze and understand the interchange of individual and gene fluxes among different populations. Thus, the present work aimed at studying the genetic variability of P. megistus in the State of Paraná - south of Brazil- and at comparing it with populations of the same species from five other states in Brazil (SP, MG, SC, RS, SE). In order to attain the proposed objective, 25 populations were studied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Panstrongylus megistus; Isoenzymes; Genetic variability; Triatomines. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200012 |
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| Penha,Tânia Regina; Krüger,Ernesto Renato; Thomaz-Soccol,Vanete; Agottani,Jorge Victor Bacila; Itano,Flávio Hiroshi; Troiano,Ludmilla Della Coletta; Brodzinski,Josiane. |
| The aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG). Out of seven hybridomas, two were chosen based on the ELISA'S absorbance values and were labeled B4F11 and B3H12. These monoclonals were analyzed through Western Blot for IgG fragments obtained by proteolysis with papain, separated by electrophoresis in polyacrylamide gel electrophoresis with β-mercaptoetanol as reducing agent. This revealed that, possibly, the B4F11 was directed to a conformational antigen, and that B3H12 reacted in a specific fashion with Fc (Bovine IgG crystallizable fragment). This antibody could be used in the development of reagents to immunoassays relevant for research and diagnosis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monoclonal antibodies; Bovine immunoglobulin G; Fragment Fc. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100014 |
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| Teodoro,Ueslei; Thomaz-Soccol,Vanete; Kühl,João B.; Santos,Demilson Rodrigues dos; Santos,Elcio Silvestre dos; Santos,Ademar Rodrigues dos; Abbas,Milia; Dias,Alessandra de Cassia. |
| The objective of the present work was to evaluate the impact of the reorganization and cleaning of the peridomicily seeking the control of Phlebotominae vectors of Leishmania. Collections of insects were made in domestic animal shelters and in peridomiciliar area located in Jussara farm, Municipality of Jussara, State of Parana, Brazil where cutaneous leishmaniasis has been endemic. The collections were accomplished five nights for month of the 21:00pm for fie hours, in 1996 and 1997. The results were compared the those obtained in the same places in 1992 and 1994. After the collections of 1992 peridomiciliar area was cleaned and organic matter was removed and domestic animals were reallocated. In 1992, 1994 and 1996/97, 10, 7 and 12 Phlebotominae species... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phlebotominae control; Leishmaniasis control; Lutzomyia whitmani; Cutaneous leishmaniasis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200007 |
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| Thomaz-Soccol,Vanete; Souza,Felipe Pohl de; Sotomaior,Cristina; Castro,Edilene Alcântara; Milczewski,Viviane; Mocelin,Giovani; Silva,Maria do Carmo Pessoa e. |
| The fecal egg count reduction test (FECRT) was used in a survey for anthelmintic resistance in 42 sheep farms, in five different regions in Paraná State, Brazil between July 1996 - July 2000. Animals with natural infection were tested. Five drugs were used individually or in combination - benzimidazole, imidazothiazole, ivermectin, milbemicina and closantel. Results showed that the prevalence of resistance to all anthelmintic was as high as 88.1% for benzimidazoles (oxfendazole), 78.6% for ivermectin, 56.4% for closantel, 38.7% for closantel + oxfendazole, 38% for levamisole, and 23.6% for moxidectin. There was multiple resistance in all tested farms. The most important infective larvae recovered from culture were Haemonchus contortus and Trichostrongylus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gastrointestinal parasite; Sheep nematode; Ovis aries; Anthelmintic resistance; FECRT. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100006 |
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| Minozzo,João Carlos; Thomaz-Soccol,Vanete; Olortegui,Carlos Chaves; Soares,Vando Edésio; Costa,Alvimar José da. |
| Um teste de ELISA indireto (ENZYME-LINKED IMMUNOSORBENT ASSAY) foi desenvolvido para pesquisa de anticorpos contra-C. bovis em bovinos experimental e naturalmente infectados. Foram estudados três antígenos: antígeno parcial de C. cellulosae, antígeno total de C. bovis e antígeno total de C. longicollis. Na padronização do ELISA foram analisadas as seguintes combinações: antígeno 250 e 500ng de proteína/cavidade, diluição dos soros 50, 100, 200 e 400 vezes, diluição do conjugado (IgG de cabra anti-IgG bovina conjugada com peroxidase) 400 e 800 vezes. Do cruzamento das condições acima resultou a seguinte padronização: antígeno 250ng/cavidade, soro e conjugado diluídos 100 e 400 vezes, respectivamente. O nível de corte (cut-off) da reação entre animais... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Imunodiagnóstico; Cisticercose bovina; Antígeno homólogo; Antígeno heterlólogo. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300031 |
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| Bertol,Melina Andrea Formighieri; Weiss,Romildo Romualdo; Thomaz-Soccol,Vanete; Kozicki,Luiz Ernandes; Fujita,Aline Silva; Abreu,Renata Azevedo de; Green,Kerriel Thandile. |
| The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p<0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bull; Epididymis; Orchiectomy; Sperm; Room temperature; Viability. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500008 |
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| Registros recuperados: 14 | |
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